SPR molecular interaction instrument


1 Model：SPR-T200, SPR-T210, SPR-I300

2 Specifications：
Model SPR-T200 SPR-T210 SPR-I300
Light Source Type He-Ne Laser He-Ne Laser He-Ne Laser,and LED
wavelength 632.8nm 632.8nm 632.8nm,and 621±10nm
Rotation Stage Number of Rotation stage Motor 1, sample motor
Motor 2, detector motor		 Motor 1, sample motor
Motor 2, detector motor		 Motor 1, sample motor
Motor 2, detector motor
Control of rotation stage Automatically control Automatically control Automatically control
Rotation angular resolution <= 0.02°
upgradable		 0.01°
upgradable		 0.01°
upgradable
Rotation angles 0-360°(Motor 1)
0-180°(Motor 2)		 0-360°(Motor 1)
0-180°(Motor 2)		 0-360°(Motor 1)
0-180°(Motor 2)
Optimal measurable angles 15-80°(incident angle)
(for prism 1.845)		 15-80°(incident angle)
(for prism 1.845)		 15-80°(incident angle)
(for prism 1.845)
Anti-collision system 2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. 2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°. 2 limit switches AZ-721, used to switch off the detector motor when it runs beyond 0-180°.
Angular calibration Semi-automatic Semi-automatic Semi-automatic
Stage Motion Type Manual Manual Manual
Translation stage XYZ mobile platform XYZ mobile platform XYZ mobile platform
Detector Photo detector Photo-diode detector Photo-diode detector A photo-diode detector, and a CCD camera
Flow Cell System Injection system Peristaltic Pump Peristaltic Pump Peristaltic Pump
Type BT100-2J BT100-2J BT100-2J
Pump channels 2 2 2 or 4(Optional)
Injection Control Both Automatic and Manual Both Automatic and Manual Both Automatic and Manual
Injection Volume 20-750 μl 20-750 μl 20-750 μl
Flow Cell Capacity 20-100 μl 20-100 μl 20-100 μl
Flow Cell Number Single Channel Single Channel 1-4 Channels (Optional）
Basic Parameters Instrument Size 1120 mm*560 mm*560 mm 1120 mm*560 mm*500 mm 1100 mm*560 mm*500 mm
Instrument Weight About 120 kg About 120 kg About 120 kg
Sensitivity 10-5-10-6RIU-1
Depends on the sensor chips		 10-5-10-6RIU-1
Depends on the sensor chips		 10-5-10-6RIU-1
Depends on the sensor chips
Accessories Prism Refractive index, 1.745 or 1.845
Exchangeable		 Refractive index, 1.745 or 1.845
Exchangeable		 Refractive index, 1.745 or 1.845
Exchangeable
Flow cell Quartz and PDMS Quartz and PDMS Quartz and PDMS
Tubes 2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm		 2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm		 2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm
Matching Oil 1.7000±0.0002
Cargille Labs		 1.7000±0.0002
Cargille Labs		 1.7000±0.0002
Cargille Labs
Sample Chips Sensor chips with 50 nm Au
Customizable		 Sensor chips with 50 nm Au
Customizable		 Sensor chips with 50 nm Au
Customizable
Others Power Supply 220V/50 HZ AC 220V/50 HZ AC 220V/50 HZ AC
Software Sensby SPR Sensby SPR Sensby SPR
System Windows 7, 10 Windows 7, 10 Windows 7, 10

3 Functions of SPR
The instrument SPR is based on Surface Plasmon Resonance (SPR) technology to monitor the interaction of biomolecules in real time. The molecular interactions information is obtained by measuring the angular reflection spectroscopy at a fixed wavelength; or measuring the kinetic of molecular interactions through reflectivity intensity changes at a fixed incident angle. SPR is able to perform the determination of kinetic constant, detection of sample concentration, analysis of the interaction modes and functional analysis of complex.

4 Main Application Field of SPR
Proteomics Immunoassay
Drug Discovery Vaccine Development
Cancer Research Transient Binding
Cell Signaling Ligand Fishing
Multi-molecular Complexes Binding Specificity
Molecular Recognition Structure-function Relationship
Immune Regulation Enzyme Reaction

5 Principle

As shown in the figure, the laser at the wavelength of 632.8 nm passes through a polarizer to become a transverse magnetically polarized beam and is coupled to glass prism to excite surface plasmon resonance of sensor chip. The reflected light intensity R is detected in real time by the changes of incident light angle θ. The detection of molecular interactions can be achieved by the changes of ΔR or Δθ; the molecular interaction kinetic constants can also be obtained by measuring the reflectivity changes at a fixed angle.

6 Data download
SPR T200 software

Model SPR-T210 SPR-I400
Detector Photo detector Photo-diode detector CCD
Flow Cell System Injection system Peristaltic Pump Peristaltic Pump
Injection Control Semi-Automatic Automatic
Sampling -- 96 hole plate
Automatic switching
Injection Volume 20-750 μl 20-750 μl
Flow Cell Capacity 20-100 μl 20-100 μl
Flow Cell Number Single Channel 4 Channels
Basic Parameters Instrument Size 1120 mm*560 mm*500 mm 850 mm*630 mm*611 mm
Instrument Weight about 120 kg about 50 kg
Measurable Sample Refractive Index 1~1.5 1~1.5
Sensitivity ~10-6 RIU
Depends on the sensor chips		 ~10-6 RIU
Depends on the sensor chips
Sensitivity for Concentration Detection 10 ng/ml 10 ng/ml
Detection Range 0.1 μg/ml – 5 mg/ml 0.1 μg/ml – 5 mg/ml
Detection Range 0.05 nM-1 mM 0.05 nM-1 mM
KD Range 0.05 nM-0.1 mM 0.05 nM-0.1 mM
Accessories Prism Refractive index, 1.845
Exchangeable		 Refractive index, 1.845
Exchangeable
Flow cell Quartz and PDMS Quartz and PDMS
Tubes 2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm		 2 Stops, Tygon R3607,
ID:0.64mm; Wall:0.91mm
Matching Oil 1.7000±0.0002
Cargille Labs		 1.7000±0.0002
Cargille Labs
Sample Chips Sensor chips with 50 nm Au
Customizable		 Sensor chips with 50 nm Au
Customizable
Others Power Supply 220V/50 HZ AC 220V/50 HZ AC
Software Sensby SPR Sensby SPR
System Windows 7, 10 Windows 7, 10